Centrifuge:
it is a process in which components are separate on basis of size ,shape, length, density ( in other words its is a separation process of material).its is a machine which is use in science laboratory such as biotechnology lab.
in this apparatus DNA ,RNA, amino acids and protein are separated by plant or animals cell or tissue (bacterial genetic material also be separated).
Principal:
Its works on density of components desire to separate in form of homogeneous solution
F=Mw²r
F=intensity of centrifugal force
M= Mass of particle
w= angular velocity of rotation
r=distance of migrating particle from center of axis of rotation
Types of Centrifuges:
1.small bench top extractors
a).in this machine without chill solution can be separated.
b).blood ,serum etc. are separated
c).slow in speed up to 4000 rpm
d).100 Eppendorf tube are can be loaded in a time.
2.Micro Extractor:
a).2mm volume of Eppendorf tube
b).10,000 Eppendorf tube can be loaded
c).protein are separated
3.High speed seperator:
a).50,000 Eppendorf tube are loaded
b).sub. organelles are separated
c).17000 rpm speed
4.ultra high speed centrifuge:
a).90,000 Eppendorf tube are loaded.
b).chilled material are used
c).microorganisms are separated
- Rotor in extractor
- Fixed rotor
horizontal solution are in rotation and clearly pellets are separated debris are obtain
- swimming bucket rotar
its solution in vertical position in rotation and no clearly pellets are separated
